Back to Article

  Legend

Fig. 5.    Reverse-phase HPLC analysis of oxidized and nontreated purified rhGAL-1. DEAE-purified rhGAL-1 was not treated further (A) or was oxidized (B) to yield the same disulfide linkages as those in rhGAL-1/COS1. Both samples (A, 0.8 µg; B, 0.6 µg) were analyzed by reverse-phase HPLC on a YMC-Pack Protein RP column (4.6 × 150 mm); elution was performed over 45 min at a flow rate of 0.5 mL·min^-1 with a linear gradient of 32–40% acetonitrile in 0.1% TFA. Protein elution was monitored by measurement of A₂₁₅.

© Blackwell Science and Munksgaard. All rights reserved. Some contents contained herein may be owned by third parties and use is prohibited without express written permission.

  technology by HealthGate Data Corp. www.healthgate.com