Abstract
The SET DOMAIN GROUP (SDG) proteins represent an evolutionarily-conserved family of epigenetic regulators present in eukaryotes and are putative candidates for the catalysis of lysine methylation in histones. Plant genomes analyses of this family have been performed in arabidopsis, maize, and rice and functional studies have shown that SDG genes are involved in the control of plant development. In this work, we describe the identification and structural characterization of SDG genes in the Vitis vinifera genome. This analysis revealed the presence of 33 putative SDG genes that can be grouped into different classes, as it has been previously described for plants. In addition to the SET domain, the proteins identified possessed other domains in the different classes. As part of our study regarding the growth and development of grapevine, we selected eight genes and their expression levels were analyzed in representative vegetative and reproductive organs of this species. The selected genes showed different patterns of expression during inflorescence and fruit development, suggesting that they participate in these processes. Furthermore, we showed that the expression of selected SDGs changes during viral infection, using as a model Grapevine Leafroll Associated Virus 3-infected symptomatic grapevine leaves and fruits. Our results suggest that developmental changes caused by this virus could be the result of alterations in SDG expression.
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Abbreviations
- SDG:
-
SET DOMAIN GROUP
- GLRaV-3:
-
Grapevine Leafroll associated virus 3
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Acknowledgments
This work was supported by the Chilean Wine Consortium 05CTE01-03, the Fruit Consortium, 07Genoma01, Fondecyt project 1100709 and Millennium Nucleus for Plant Functional Genomics (P06-009-F). F. Aquea is supported by a Postdoctoral Project “Programa Bicentenario de Ciencia y Tecnología/CONICYT-BancoMundial” PSD74-2006. We thank Michael Handford for assistance in language support. M.J. Poupin is supported by a Postdoctoral project FONDECYT 3100040 and a CONICYT project PAI 79090016.
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Communicated by D. Zaitlin.
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Aquea, F., Vega, A., Timmermann, T. et al. Genome-wide analysis of the SET DOMAIN GROUP family in Grapevine. Plant Cell Rep 30, 1087–1097 (2011). https://doi.org/10.1007/s00299-011-1015-0
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DOI: https://doi.org/10.1007/s00299-011-1015-0