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Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke

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Book cover Neural Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1059))

Abstract

Human embryonic stem cells (hESCs) are pluripotent with a strong self-renewable ability making them a virtually unlimited source of neural cells for structural repair in neurological disorders. Currently, hESCs are one of the most promising cell sources amenable for commercialization of off-shelf cell therapy products. However, along with this strong proliferative capacity of hESCs comes the tumorigenic potential of these cells after transplantation. Thus, the isolation and purification of a homogeneous, population of neural stem cells (hNSCs) are of paramount importance to avoid tumor formation in the host brain. This chapter describes the isolation, neuralization, and long-term perpetuation of hNSCs derived from hESCs through use of specific mitogenic growth factors and the preparation of hNSCs for transplantation in an experimental model of stroke. Additionally, we describe methods to analyze the stroke and size of grafts using magnetic resonance imaging and Osirix software, and neuroanatomical tracing procedures to study axonal remodeling after stroke and cell transplantation.

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Acknowledgments

Dr Azevedo-Pereira is supported by Coordenação de Aperfeiç-oamento de Pessoal de Nível Superior (CAPES, Brazil).

Author information

Authors and Affiliations

  1. Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA

    Ricardo L. Azevedo-Pereira

  2. Department of Neurosurgery, Stanford University School of Medicine, California & NeoNeuron, Palo Alto, California, USA

    Marcel M. Daadi

Authors
  1. Ricardo L. Azevedo-Pereira
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  2. Marcel M. Daadi
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, University of Florida, Gainesville, Florida, USA

    Brent A. Reynolds

  2. Department of Neurosurgery, University of Florida, Gainesville, Florida, USA

    Loic P. Deleyrolle

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© 2013 Springer Science+Business Media New York

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Azevedo-Pereira, R.L., Daadi, M.M. (2013). Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke. In: Reynolds, B., Deleyrolle, L. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 1059. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-574-3_14

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  • DOI: https://doi.org/10.1007/978-1-62703-574-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-573-6

  • Online ISBN: 978-1-62703-574-3

  • eBook Packages: Springer Protocols

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