Editors:
- Provides an overview of the exon skipping field
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 867)
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Table of contents (27 protocols)
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Front Matter
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Tools to Induce Exon Skipping
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Front Matter
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About this book
“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
Editors and Affiliations
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Dept. Human & Clinical Genetics, Leiden University Medical Center, Leiden, Netherlands
Annemieke Aartsma-Rus
Bibliographic Information
Book Title: Exon Skipping
Book Subtitle: Methods and Protocols
Editors: Annemieke Aartsma-Rus
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-767-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media,LLC 2012
Hardcover ISBN: 978-1-61779-766-8Published: 28 March 2012
Softcover ISBN: 978-1-4939-6206-8Published: 23 August 2016
eBook ISBN: 978-1-61779-767-5Published: 28 March 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 440
Number of Illustrations: 62 b/w illustrations, 8 illustrations in colour
Topics: Human Genetics