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Adaptation to freshwater in the palaemonid shrimp Macrobrachium amazonicum: comparative ontogeny of osmoregulatory organs

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Abstract

The ontogeny of osmoregulatory organs was studied in two geographically isolated populations of the palaemonid shrimp Macrobrachium amazonicum, one originating from the Amazon estuary (A) and the other from inland waters of the Pantanal (P) in northeastern and southwestern Brazil, respectively. A previous investigation had shown that the estuarine population is able to hypo-osmoregulate in seawater, whereas the hololimnetic inland population has lost this physiological function. In the present study, the structural development of the branchial chamber and excretory glands and the presence of Na+/K+-ATPase (NKA) were compared between populations and between larval and juvenile stages after exposure to two salinities representing hypo- and hypertonic environments. In the newly hatched zoea I stage of both populations, gills were absent and NKA was localized along the inner epithelium of the branchiostegite. In intermediate (zoea V) and late larval stages (decapodids), significant differences between the two populations were observed in gill development and NKA expression. In juveniles, NKA was detected in the gills and branchiostegite, with no differences between populations. At all developmental stages and in both populations, NKA was present in the antennal glands upon hatching. The strong hypo-osmoregulatory capacity of the early developmental stages in population A could be linked to ion transport along the inner side of the branchiostegite; this seemed to be absent or weak in population P. The presence of fully functional gills expressing NKA appears to be essential for efficient hyper-osmoregulation in late developmental stages during successful freshwater adaptation and colonization.

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Acknowledgments

The authors are grateful to Dr. Liliam Hayd, State Univertsity of Mato Grosso do Sul, Aqudauana, Brazil, for transporting live shrimp from Brazil to Helgoland, to IBAMA (Brasília) for the permit to export shrimps, to Uwe Nettelmann for help in maintaining shrimp cultures and to Maryline Bossus for her advice.

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Authors and Affiliations

  1. Université Montpellier 2, Equipe Adaptation Ecophysiologique et Ontogénèse, UMR5119 EcoSyM, UM1-2-CNRS-IRD-Ifremer, cc 092, Place E. Bataillon, 34095, Montpellier cedex 05, France

    Nesrine Boudour-Boucheker, Viviane Boulo, Catherine Lorin-Nebel, Camille Elguero, Evelyse Grousset, Mireille Charmantier-Daures & Guy Charmantier

  2. Alfred-Wegener-Institut, Biologische Anstalt Helgoland, 27498, Helgoland, Germany

    Klaus Anger

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  1. Nesrine Boudour-Boucheker
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  2. Viviane Boulo
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  3. Catherine Lorin-Nebel
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  4. Camille Elguero
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  5. Evelyse Grousset
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  6. Klaus Anger
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  7. Mireille Charmantier-Daures
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  8. Guy Charmantier
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Correspondence to Nesrine Boudour-Boucheker.

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This work was partially funded by the Algerian Ministry of Higher Education and Scientific Research (MESRS/CNEPRU).

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Boudour-Boucheker, N., Boulo, V., Lorin-Nebel, C. et al. Adaptation to freshwater in the palaemonid shrimp Macrobrachium amazonicum: comparative ontogeny of osmoregulatory organs. Cell Tissue Res 353, 87–98 (2013). https://doi.org/10.1007/s00441-013-1622-x

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