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Evaluation of FluoroProbe® performance for the phytoplankton-based assessment of the ecological status of Mediterranean coastal lagoons

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Abstract

The European Water Framework Directive and several other legislations worldwide have selected phytoplankton for monitoring the ecological status of surface waters. This assessment is a complicated task in coastal lagoons due to their intrinsic variability, prompting moves to use real-time measurements. Here, we tested the ability of the submersible spectrofluorometer FluoroProbe® to accurately estimate the phytoplankton biomass and to efficiently discriminate spectral groups in Mediterranean coastal lagoons, by using sub-surface water samples (n = 107) collected at Biguglia lagoon (Corsica) in different environmental situations (salinity and trophic state) from March 2012 to December 2014. We compared the estimates of biomass and phytoplankton group composition obtained with the FluoroProbe® (in situ and lab measurements) with the spectrofluorimetrically measured biomass and HPLC-derived quantifications of pigment concentrations. FluoroProbe® provided good estimates of the total phytoplankton biomass (particularly, the lab measurements). The FluoroProbe® data were significantly correlated with the HPLC results, except for the in situ measurements of very weak concentrations of blue-green and red algae. Our findings indicate that factory-calibrated FluoroProbe® is an efficient and easy-to-use real-time phytoplankton monitoring tool in coastal lagoons, especially as an early warning system for the detection of potentially harmful algal blooms. Practical instructions dedicated to non-specialist field operators are provided. A simple and efficient method for discarding in situ measurement outliers is also proposed.

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Acknowledgements

The authors would like to thank the two anonymous reviewers for their constructive comments that improved our manuscript.

Funding

This study was supported by funding from the French Government and from the Corsican Regional Council. MG was awarded a grant from the Collectivité Territoriale de Corse and the Université de Corse.

Author information

Authors and Affiliations

  1. UMR SPE CNRS, UMS Stella Mare CNRS, Université de Corse, BP 52, 20250, Corte, France

    Marie Garrido & Vanina Pasqualini

  2. MARBEC, CNRS, Ifremer, IRD, University Montpellier, Montpellier, France

    Philippe Cecchi & Béatrice Bec

  3. Centre de Recherches Océanologiques (CRO), BP V18, Abidjan 18, Côte d’Ivoire

    Philippe Cecchi

  4. IFREMER, Laboratoire Environnement Ressources Provence-Azur-Corse, Station de Corse, Z.I. Furiani, Immeuble Agostini, 20600, Bastia, France

    Nathalie Malet

  5. Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, UMR IMBE, Aix Marseille Univ, Avignon Université, CNRS, IRD, Marseille, France

    Franck Torre

Authors
  1. Marie Garrido
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  2. Philippe Cecchi
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  3. Nathalie Malet
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  4. Béatrice Bec
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  5. Franck Torre
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  6. Vanina Pasqualini
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Corresponding author

Correspondence to Philippe Cecchi.

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Garrido, M., Cecchi, P., Malet, N. et al. Evaluation of FluoroProbe® performance for the phytoplankton-based assessment of the ecological status of Mediterranean coastal lagoons. Environ Monit Assess 191, 204 (2019). https://doi.org/10.1007/s10661-019-7349-8

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  • DOI: https://doi.org/10.1007/s10661-019-7349-8

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