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Impacts of bioremediation schemes for the mitigation of a low-dose anthracene contamination on free-living marine benthic nematodes

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Abstract

A microcosm experiment was used to examine (1) the effects of different bioremediation schemes on degradation of anthracene and the structure of free-living marine nematodes in a lightly contaminated (4.5 μg g−1) sediment from Bizerte lagoon and (2) the responses of the nematode community upon an artificial spiking of a low dose anthracene (1 μg g−1). For that purpose sediment microcosms were incubated in laboratory for 40 days. Bioremediation techniques decreased the anthracene contamination, and interestingly, biodegradation were more efficient when anthracene was artificial supplied into the sediment suggesting that the addition of bioavailable anthracene stimulated the bacterial community to adjust towards a PAH-degrading community. Spiking with this low dose of anthracene provoked significant changes in the nematode community structure and abundance, with the elimination of specific species such as Mesacanthion diplechma, the decrease of the dominant species Oncholaimus campylocercoides and the increase in abundance of opportunistic species such as Spirinia parasitifera. This would suggest a low tolerance of the nematode community despite the presence of a weak anthracene contamination in the sediment that could have allow dominance of an anthracene tolerant nematode species. Anthracene toxicity was alleviated in biostimulation treatments, leading to a strong increase in nematode abundance, concomitantly with changes in the nematode community structure; Prochromadorella neapolitana became the most abundant species.

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Acknowledgments

This work was supported by funding from the CMCU program (PHC-UTIQUE, n° 09G 0189), Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD) and the Faculté des Sciences de Bizerte (FSB). Dr. Emma Rochelle-Newall is gratefully acknowledged as native English speaker for her helpful criticisms on an early version of the manuscript and for English improvements.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire de Biosurveillance de l’Environnement, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia

    Hela Louati, Olfa Ben Said, Amel Soltani, Patricia Aissa, Ezzeddine Mahmoudi & Olivier Pringault

  2. Equipe Environnement et Microbiologie, UMR CNRS IPREM 5254 IBEAS, Université de Pau et des Pays de l’Adour, Avenue des Facultés, 64000, Pau Cedex, France

    Olfa Ben Said, Amel Soltani, Cristiana Cravo-Laureau & Robert Duran

  3. Laboratoire Ecosystèmes Marins Côtiers, UMR 5119 ECOSYM CNRS-IRD-UM2-IFREMER, Université Montpellier 2, Place Eugène Bataillon, Case 093, 34095, Montpellier Cedex, France

    Hela Louati & Olivier Pringault

  4. Laboratoire de Chimie Analytique, Bio-inorganique et Environnement UMR CNRS IPREM 5254, Université de Pau et des Pays de l’Adour, 2 Avenue Angot, 64053, Pau Cedex, France

    Hugues Preud’Homme

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  1. Hela Louati
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  2. Olfa Ben Said
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Louati, H., Ben Said, O., Soltani, A. et al. Impacts of bioremediation schemes for the mitigation of a low-dose anthracene contamination on free-living marine benthic nematodes. Ecotoxicology 23, 201–212 (2014). https://doi.org/10.1007/s10646-013-1163-9

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