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Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality

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Abstract

Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.

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Acknowledgements

This study was conducted as part of the European program “Aquafirst” and the network of excellence “Marine Genomic Europe.” The authors are grateful to J. Lafore, V. Quillien, G. Davey, and P. Lindeque for their support during the course of this work. We are indebted to Helen McCombie for her help in editing the English language. We also thank P. Prunet, A. Le Cam, S. Mativet, and G. Leborgne from INRA-SCRIBE for excellent technical assistance and the SIGENAE team (INRA Toulouse, France) for bioinformatic tool development (http://www.sigenae.org). We acknowledge the Max Planck Institute of Berlin (Germany) for the preparation of the slides. E. Fleury was funded by Ifremer and a Région Basse Normandie doctoral grant.

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Authors and Affiliations

  1. UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”, Centre de Brest, B.P. 70, 29280 Plouzané/IBFA, IFR ICORE 146, 14032, Caen, France

    Elodie Fleury, Jeanne Moal, Jean-Yves Daniel, Charlotte Corporeau, Pierre Boudry, Pascal Favrel & Arnaud Huvet

  2. Ifremer, CNRS Université de Montpellier 2, UMR 5119 ECOLAG CC 80, Place Eugène Bataillon, 34095, Montpellier, France

    Viviane Boulo

  3. Ifremer, UMR 1067 Nutrition, aquaculture, et génomique, Centre de Brest, B.P. 70, 29280, Plouzané, France

    David Mazurais

  4. CNRS Université Pierre et Marie Curie, UMR 7138 Systématique, adaptation, évolution, Case 05, 75252, Paris, France

    Alain Hénaut

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  1. Elodie Fleury
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  2. Jeanne Moal
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  3. Viviane Boulo
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  4. Jean-Yves Daniel
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  6. Alain Hénaut
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  7. Charlotte Corporeau
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  10. Arnaud Huvet
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Correspondence to Arnaud Huvet.

Appendix

Appendix

Complete list of the 2,114 Crassostrea gigas ESTs varying significantly according to the factor “time” (dates 1, 2, 3, and 4), independent of the resistant or sensitive line.

“GenBank #” corresponds to the GenBank accession number, “Sigenae Name” corresponds to the ESTs names available in the GigasDatabase (http://public-contigbrowser.sigenae.org:9090/Crassostrea_gigas/index.html), and “p value ANOVA” corresponds to the p value obtained with the GeneANOVA analysis.

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Fleury, E., Moal, J., Boulo, V. et al. Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality. Mar Biotechnol 12, 326–339 (2010). https://doi.org/10.1007/s10126-009-9227-9

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