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The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax

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Abstract

Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-α1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-α1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.

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Acknowledgments

We wish to thank the Creufop at the Marine Biological Station of Sète for providing sea bass juveniles and Evelyse Grousset for technical help. We thank Julien Issartel and Erick Desmarais for their judicious advices, and also anonymous reviewers for their contributions to the improvement of the manuscript. Data used in this work were produced through the technical facilities of the Centre Méditerranée Environnement Biodiversité and the platform qPCR Haut Débit of the IFR122. Pictures of immunostaining were produced through the Montpellier RIO Imaging platform. Maryline Bossus thanks the French government—Ministry of Research and Technology—for her ATP (“Action Thématique Prioritaire”) Doctorate scholarship.

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  1. Equipe Adaptation Ecophysiologique et Ontogenèse, UMR5119 - EcoSyM, UM2-UM1-CNRS-IRD-IFREMER, cc 092, Place E. Bataillon, 34095, Montpellier cedex 05, France

    Maryline Bossus, Guy Charmantier, Eva Blondeau-Bidet, Bianca Valletta, Viviane Boulo & Catherine Lorin-Nebel

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  1. Maryline Bossus
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  2. Guy Charmantier
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  3. Eva Blondeau-Bidet
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  4. Bianca Valletta
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  5. Viviane Boulo
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  6. Catherine Lorin-Nebel
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Correspondence to Maryline Bossus.

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Communicated by G. Heldmaier.

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Bossus, M., Charmantier, G., Blondeau-Bidet, E. et al. The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax . J Comp Physiol B 183, 641–662 (2013). https://doi.org/10.1007/s00360-012-0737-9

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  • DOI: https://doi.org/10.1007/s00360-012-0737-9

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