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Isolation and characterisation of 19 microsatellite loci from the Amazonian frog Adenomera andreae (Amphibia: Anura: Leptodactylidae)

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Abstract

Nineteen novel microsatellite loci were isolated from Adenomera andreae, a widespread Amazonian frog considered to be a species complex. Three multiplex kits were optimized. Genetic diversity was assessed in 66 individuals sampled in three populations along the West of the Approuague River catchment (French Guiana). We also tested the multiplex kits in four other Adenomera and nine Leptodactylus species with 43.4 and 17.5% success respectively.

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Acknowledgments

This work has been supported by a grant to A.F. by Centre National de la Recherche Scientifique “Amazonie” program. Data used in this work were mostly produced through molecular genetic analysis technical facilities of the Institut National de la Recherche Agronomique Kourou and IFR119 “Montpellier Environnement Biodiversité”. We are indebted toward Philippe Gaucher and Michel Blanc for helping with field work organization. Field work has also been helped by Alec Baxt, Partick Chatelet, Dirk Schmeller, Fabrice Hibert, Aurélie Condevaux, Max Ringler, Eva Ursprung and Stéphane Icho. Lab work benefited from the experience of Valérie Troispoux and Eliane Louisianna.

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Authors and Affiliations

  1. CNRS, IRD, UMR 6116 – IMEP, Equipe Persistance et Evolution de la Biodiversité, Centre Saint-Charles, Aix-Marseille Université, Case 36, 3 Place Victor Hugo, 13331, Marseille Cedex 3, France

    Antoine Fouquet & Vincent Dubut

  2. Department of Biology, University of Mississippi, Box 1848, Oxford, MS, 38677, USA

    Robert A. Hataway & Brice P. Noonan

  3. INRA - UMR ECOFOG, Campus Agronomique – Avenue de France, BP 709-97387, Kourou, French Guiana, France

    Caroline Scotti-Saintagne & Ivan Scotti

Authors
  1. Antoine Fouquet
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  2. Vincent Dubut
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  3. Robert A. Hataway
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  4. Caroline Scotti-Saintagne
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  5. Ivan Scotti
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  6. Brice P. Noonan
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Correspondence to Antoine Fouquet.

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Fouquet, A., Dubut, V., Hataway, R.A. et al. Isolation and characterisation of 19 microsatellite loci from the Amazonian frog Adenomera andreae (Amphibia: Anura: Leptodactylidae). Conservation Genet Resour 1, 217 (2009). https://doi.org/10.1007/s12686-009-9053-4

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  • DOI: https://doi.org/10.1007/s12686-009-9053-4

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