Skip to main content
Log in

Isolation of primers for candidate genes for mechano-sensing in five Neotropical tree species

  • Short Communication
  • Published:
Tree Genetics & Genomes Aims and scope Submit manuscript

Abstract

Mechanical signals have an impact on plant development. Tropical rainforest trees display large variability for life–history traits related to biomechanics and therefore are a unique study system to better understand biomechanical trait variability from an evolutionary perspective. From sequences and gene expression data available in model species, we developed specific primers for six candidate genes for mechano-sensing in five tropical species. Most of the gene sequences were polymorphic in most species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • APG (2003) An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG II. Bot J Linn Soc 141:399–436

    Article  Google Scholar 

  • Audigeos D, Buonamici A, Belkadi L, Boshier DH, Scotti-Saintagne C, Vendramin G, Scotti I (2010) Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species. BMC Evol Biol 10:202–219

    Article  PubMed  Google Scholar 

  • Azri W, Chambon C, Herbette S et al (2009) Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation. Physiol Plant 136:193–208

    Article  PubMed  CAS  Google Scholar 

  • Colpaert N, Cavers S, Bandou E et al (2005) Sampling tissue for DNA analysis of trees: trunk cambium as an alternative to canopy leaves. Silvae Genet 54:265–269

    Google Scholar 

  • Coutand C, Chevolot M, Lacointe A, Rowe N, Scotti I (2010) Mechanosensing of stem bending and its interspecific variability in five neotropical rainforest species. Ann Bot 105:341–347

    Article  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull 19:11–15

    Google Scholar 

  • Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704

    Article  PubMed  Google Scholar 

  • Hall T (1999) BioEdit: a user-friendly biological sequence alignment and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Jaouen G, Alméras T, Coutand C, Fournier M (2007) How to determine sapling buckling risk with only a few measurements. Ann Bot 94:1583–1593

    Google Scholar 

  • Keane TM, Naughton TJ, McInerney JO (2004) Modelgenerator: amino acid and nucleotide substitution model selection. National University of Ireland. available at: http://bioinf.nuim.ie/software/modelgenerator.

  • Küppers M (1989) Ecological significance of aboveground architectural patterns in woody plants: a question of cost-benefit relationships. Trends Ecol Evol 4:375–379

    Article  PubMed  Google Scholar 

  • Lee D, Polisensky DH, Braam J (2005) Genome-wide identification of touch- and darkness regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes. New Phytol 165:429–444

    Article  PubMed  CAS  Google Scholar 

  • Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452

    Google Scholar 

  • Malo MS, Srivastava K, Andresen JM, Chen XN, Korenberg JR, Ingram VM (1994) Targeted gene walking by low stringency polymerase chain reaction: assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31. Proc Natl Acad Sci USA 91:2975–2979

    Article  PubMed  CAS  Google Scholar 

  • Martin L, Leblanc-Fournier N, Azri W et al (2009) Characterization and expression analysis under bending and other abiotic factors of PtaZFP2, a poplar gene encoding a Cys2/His2 zinc finger protein. Tree Physiol 29:125–136

    Article  PubMed  CAS  Google Scholar 

  • Moulia B, Coutand C, Lenne C (2006) Posture control and skeletal mechanical acclimation in terrestrial plants: implications for mechanical modeling of plant architecture. Am J Bot 93:1477–1489

    Article  Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Google Scholar 

  • Purugganan MM, Braam J, Fry SC (1997) The Arabidopsis TCH4 xyloglucan endotransglycosylase’. Plant Physiol 115:181–190

    Article  PubMed  CAS  Google Scholar 

  • Rozen S, Skaletski HJ (2000) Primer3 on the WWW for general use and for biologist programmers. Code available at http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi.

  • Stephens M, Smith N, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989

    Article  PubMed  CAS  Google Scholar 

  • Telewski FW (2006) A unified hypothesis of mechanoperception in plants. Am J Bot 93:1466–1476

    Article  Google Scholar 

  • van Gelder HA, Poorter L, Sterck FJ (2006) Wood mechanics, allometry, and life-history variation in a tropical rain forest tree community. New Phytol 171:367–378

    Article  PubMed  Google Scholar 

  • Watterson GA (1975) On the number of segregating sites in genetical models without recombination. Theor Popul Biol 7:256–276

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work has been supported by the Woodiversity grant (Agence Nationale de la Recherche). The authors wish to thank Meriem Fournier, Nick Rowe, and Bruno Moulia for useful discussions, and Jean Weigel and Saint-Omer Cazal for technical help.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ivan Scotti.

Additional information

Communicated by S. González-Martínez

Electronic supplementary materials

Below is the link to the electronic supplementary material.

ESM 1

(DOC 582 kb)

ESM 2

(PDF 97 kb)

ESM 3

(PDF 230 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chevolot, M., Louisanna, E., Azri, W. et al. Isolation of primers for candidate genes for mechano-sensing in five Neotropical tree species. Tree Genetics & Genomes 7, 655–661 (2011). https://doi.org/10.1007/s11295-010-0363-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11295-010-0363-1

Keywords

Navigation