Elsevier

Forest Ecology and Management

Volume 257, Issue 1, 20 January 2009, Pages 351-360
Forest Ecology and Management

Structure of aquatic insect communities in tank-bromeliads in a East-Amazonian rainforest in French Guiana

https://doi.org/10.1016/j.foreco.2008.09.010Get rights and content

Abstract

Tank-bromeliads are discrete habitats which contain distinct aquatic communities, and which commonly occur in the neotropics. Because they span a broad range of ecological gradients in terms of habitat structure and amount of resources, researchers study the associations between the biodiversity of communities and these gradients in rainforests, where the very high species richness and densities within other continuous habitats makes it difficult to quantify animal communities. We analysed the diversity of aquatic insect communities in relation to different tank-bromeliad species in a primary rainforest (French Guiana) using artificial intelligence and complex optimization techniques to classify communities and model their determinants. First, the self-organizing map (neural network) was used to classify 158 bromeliads according to the quantitative structure of the insect communities. Catopsis berteroniana and Guzmania lingulata formed separate clusters of plants on the virtual map, while Vriesea splendens, Vriesea pleiosticha and Aechmea melinonii were grouped together in the remaining clusters. Some insect taxa occurred in all bromeliads, while other taxa were specific to a given species. Second, general linear modelling allowed us to specify the influence of the bromeliad species; water volume and volume of fine particulate organic matter inside of the tank; elevation above the ground; and sampling site on taxonomic richness and insect abundance. The number of taxa and individuals per plant increased with greater water volume, but the slope of the relationships depended on the bromeliad species. The significant influence of bromeliad species suggested that at similar water volumes different plant species had different taxon richness and insect abundance. Greater amounts of fine particulate organic matter were detrimental to community diversity in the tanks, probably because they decreased available space by clogging it and/or affected oxygen concentrations. The influence of tank-bromeliad species on the aquatic insect community was primarily related to their physical (and probably chemical) features rather than to species-specific associations sensu stricto. The classification of bromeliads with respect to animal species is likely to provide referential schemes for those biodiversity patterns to be expected under certain conditions, and may help to target model communities for subsequent experimental research.

Introduction

Human impact on the environment obviously causes a decline in biological diversity from a local to a global scale (Brooks and Kennedy, 2004). In this context, a major challenge for ecologists is to understand the structure and dynamics of biological communities in relation to environmental variability (Gaston, 2000). Whilst they only represent 6–7% of continental surfaces, tropical rainforests shelter more than half of the Earth’s species (Raven, 1988, Wilson, 1988). Thus, deforestation rates in the humid tropics provide compelling reasons to conduct detailed investigations of community diversity (Bowles et al., 1998, DeVries et al., 1997) for evidence of biological responses to habitat characteristics and/or degradation (Yanoviak et al., 2006). For instance, classifying sites of high biological quality is likely to provide system-specific predictions of the fauna and/or flora to be expected under undisturbed conditions. However, studying community patterns in tropical ecosystems is complicated by two factors. First, taxonomic information is limited, and most species remain undescribed (Godfray et al., 1999). Second, the very high species densities within continuous habitats makes it difficult, if not impossible, to exhaustively sample and, thus, quantify animal communities. To overcome this problem, some authors have focussed on natural microcosms with discrete boundaries such as small water tanks (Richardson, 1999, Kitching, 2001, Srivastava et al., 2004, Yanoviak, 2001).

Many species of Bromeliads (Bromeliaceae) have tightly interlocking leaves forming wells (or phytotelmata) that hold rainwater. These monocotyledon plants are represented by 1500–3000 species, and are mostly restricted to the neotropics and Florida (there is also one naturally occurring African species, namely Pitcairnia feliciana). Most of them are epiphytes. Because phytotelmata collect leaf litter and dead invertebrates, they provide a habitat for aquatic species (Carrias et al., 2001) among which insect larvae are well represented (Picado, 1913, Laessle, 1961, Kitching, 2000). The detritus provides a source of nutrients for the insect community, as well as the bromeliad itself (Richardson et al., 2000, Ngai and Srivastava, 2006). Tank-bromeliads are thus discrete habitats which contain distinct aquatic communities. The main advantage of studying these natural microcosms is their abundance in any given area. Moreover, owing to their small size, the entire aquatic community from each plant can be quantified with a degree of accuracy not possible in larger systems (Richardson, 1999). Because many aquatic insects complete their larval development in tank-bromeliads, differences in plant morphology may play an important role in habitat selection by ovipositing adults. More specifically, co-occurring bromeliads show a high variability in terms of colour patterns, size, reservoir shape and volume. Bromeliads with large central pools (e.g., Neoregelia sp.) were found to differ in their species assemblages from those with many separate, smaller pools (e.g., Guzmania sp.) (Harris, 1993). Armbruster et al. (2002) found that both biological interactions (i.e., competition and predation) and abiotic factors (the physical structure of the plant, water volume) influenced the species richness of bromeliad-inhabiting communities. However, they did not consider the different bromeliad species separately. To the best of our knowledge, there is nothing in the published literature to demonstrate that there have been previous attempts to classify individual tank-bromeliads according to their aquatic biota (see review of key studies on bromeliad communities in Kitching (2000)), and there is no evidence of species-specific associations between tank-bromeliad species and particular sets of aquatic animals (Benzing, 1990). Therefore, the existence of such associations must be tested by classifying different species of bromeliads according to the structure of their aquatic communities. Furthermore, classifying tank-bromeliads based on their biota is a crucial step towards optimizing the design of new surveys (e.g., biomonitoring) and/or experiments (hypothesis testing on some targeted insect communities), while improving our understanding of the dynamics of biodiversity in tropical habitats.

The aim of our study was to assess the relationships between tank-bromeliad species and the quantitative structure of aquatic insect communities in a primary rainforest in French Guiana. To this end, 158 plants belonging to five species were sampled at four distinct sites. The aquatic insects were mostly keyed to morphotypes and enumerated. Ecological data such as species abundances often vary and covary in a nonlinear fashion (Lek and Guégan, 2000), depending on the distribution of sampling locations. Thus, non-linear modelling methods such as artificial neural networks (ANNs) should theoretically be preferred for dealing with such data (Blayo and Demartines, 1991). Combining clustering and ordering abilities, the self-organizing map algorithm (SOM, unsupervised neural network; Kohonen, 2001) is a powerful tool used to visualize high-dimensional data. This technique has had particular relevance in revealing patterns of biological communities in relation to environmental characteristics because the gradient distribution of the biological variables can be visualized in a two-dimensional map (Park et al., 2003), and because it is particularly appropriate for dealing with outlying and rare species. We used the SOM algorithm to interpret the variability of aquatic insect communities with respect to bromeliad species. Self-organizing maps and other ANN techniques have been successfully implemented in various aspects of ecological modelling such as classifying groups (Levine et al., 1996), patterning complex relationships (Tuma et al., 1996), predicting population and community development (Recknagel et al., 1997), modelling habitat suitability (Özesmi and Özesmi, 1999), and assessing water quality (Walley et al., 2000, Céréghino et al., 2003). General linear modelling (GLM) was used to specify the influence of variables related to plant characteristics (species, volume of water and fine particulate organic matter in the tank, elevation above the ground) and site location (elevation a.s.l.) on the taxonomic richness and number of individuals per plant.

Section snippets

Study area

This study was conducted in a primary rainforest characteristic of the eastern Amazon, around the Nouragues Tropical Forest Research Station (4°5′N, 52°41′W, French Guiana, Fig. 1). The area is totally uninhabited and anthropogenic disturbance is almost completely absent. The Station is located in the Nouragues Natural Reserve, 100 km away from Cayenne and 40 km from the nearest village (Regina). The area is delineated by hills (elevation <120 m a.s.l.), and by the Balenfois mountains (maximum

Community composition and sampling adequacy

Insect abundance and taxonomic richness ranged from 1 to 112 individuals and from 1 to 9 taxa per plant, respectively. The list of taxa found in each bromeliad species is provided in Table 2. Overall, the five bromeliads studied hosted 44 aquatic insect taxa. Most taxa (30) belonged to the Diptera. Other taxa were the Heteroptera (7 morphotypes), Coleoptera (6 morphotypes), and Odonata (1 morphotype). In terms of abundance, seven taxa (Chironomini sp1, Tanypodinae, Chironomidae sp2, Wyeomyia,

Discussion

Many studies have described the diversity and/or taxonomy of bromeliad-dwelling organisms (e.g., mosquitoes, midges, crustaceans; Frank, 1983, Little and Hebert, 1996, Grogan and Hribar, 2006), but relatively few authors have actually used tank-bromeliads to analyse the diversity of simplified communities in tropical ecosystems (but see Cotgreave et al., 1993, Richardson, 1999, Armbruster et al., 2002). It should be noted, however, that research focused on other phytotelm species (notably the

Acknowledgements

We are grateful to Andrea Dejean and Peter Davies for proofreading the manuscript. This work was supported by the Programme Amazonie II of the French Centre National de la Recherche Scientifique (Project 2ID), and the Programme Convergence 2007–2013 (Région Guyane) from the European Community (Project DEGA). We wish to thank Alain G.B. Thomas for his taxonomic assistance, Marc Gibernau for his help and advice on the GLM analyses, Jérôme Orivel for providing us with Fig. 1. Two anonymous

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